rat anti mouse il 22ra1  (R&D Systems)

Journal: Atherosclerosis

Article Title: IL-22 affects smooth muscle cell phenotype and plaque formation in apolipoprotein E knockout mice.

doi: 10.1016/j.atherosclerosis.2015.08.006

Figure Lengend Snippet: Fig. 1. Expression of the IL-22 receptor in the vascular wall. Sections from the aortic root of 12 week old (A) and 24 week old (B) Apoe/ mice were stained for the IL-22RA1 subunit of the IL-22 receptor and a-actin. Positive staining is shown in brown (DAB). (C) Carotid arteries from wild type C57Bl6 mice, with our without vascular injury were stained for IL- 22RA1 or myosin heavy chain (MyHC11) using immunofluorescence. IL-22RA1 and MyHC11 are shown in red, DAPI in blue, and elastic membranes in green autofluoresence. Exclusion of primary antibody served as negative control. Scale bars represent 100 mm.

Article Snippet: For analysis of aortic root plaques, frozen tissue sections were cut from the aortic root, frozen and stained using the following primary antibodies: rat anti-mouse IL-22RA1 antibody (R&D systems, Abingdon, UK), rabbit anti-mouse SMC a-actin antibody (Abcam, Cambridge, UK), rat anti-mouse MOMA-2 antibody (BMA Biomedicals, Augst, Switzerland), rabbit-anti-mouse Caldesmon (Abcam), rabbit-anti-mouse CD68 (Abcam).

Techniques: Expressing, Staining, Negative Control